DNA
SOPP3

Part:BBa_K4138001:Design

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


Photosensitizer SOPP3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project MEtaPhos, we only expressed SOPP3 in fusion with the phosphate-binding protein 2ABH. For design considerations on them, see the corresponding composite part BBa_K4138004.

Source

We took the genomic sequence from a reference paper [1].


References

[1] Gerlach, T., et al., Photo-Regulation of Enzyme Activity: The Inactivation of a Carboligase with Genetically Encoded Photosensitizer Fusion Tags. Frontiers in Catalysis, 2022. 2. https://doi.org/10.3389/fctls.2022.835919